Preparation of BMP-2-PLLA Microspheres and Its Maintenance for Bioactivity of BMP-2

Various Dosages of BMP-2 for Management of Massive Bone Defect in Sprague Dawley Rat

Introduction: The use of BMP-2 plays an important role in the treatment of extensive bone defect. However, data about the optimal dosage of BMP-2 in the massive bone defect cases is rare. Material and Method: Twenty-five SD rats were randomly divided into a control group ofhydroxyapatite (HA) alone (Group I), HA+BMP-2 1µg/mL (Group II), HA+BMP-2 5 ug/mL (Group III), HA+BMP-2 10 µg/mL (Gro...

Gremlin1 preferentially binds to bone morphogenetic protein-2 (BMP-2) and BMP-4 over BMP-7.

Gremlin (Grem1) is a member of the DAN family of secreted bone morphogenetic protein (BMP) antagonists. Bone morphogenetic protein-7 (BMP-7) mediates protective effects during renal fibrosis associated with diabetes and other renal diseases. The pathogenic mechanism of Grem1 during diabetic nephropathy (DN) has been suggested to be binding and inhibition of BMP-7. However, the precise interacti...

Expression of Genes for Bone Morphogenetic Proteins Bmp-2, Bmp-4 and Bmp-6 in Various Parts of Human Skeleton

Fibrin Hydrogel Based Bone Substitute Tethered with BMP-2 and BMP-2/7 Heterodimers

Current clinically used delivery methods for bone morphogenetic proteins (BMPs) are collagen based and require large concentrations that can lead to dangerous side effects. Fibrin hydrogels can serve as osteoinductive bone substitute materials in non-load bearing bone defects in combination with BMPs. Two strategies to even further optimize such a fibrin based system include employing more pote...

Expression, purification and osteogenic bioactivity of recombinant human BMP-2 produced by Escherichia coli

INTRODUCTION: Among BMP family, BMP-2 is the best characterized molecule and it has the strongest bone-inducing activity. Previously, most rhBMPs have been purified mainly from mammals, like Chinese hamster ovary (CHO) cells. Problems associated with clinical application of CHO-derived recombinant human bone morphogenetic protein are post-translational problems, its low yields and high cost due...